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KMID : 1094720160210010183
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Biotechnology and Bioprocess Engineering
2016 Volume.21 No. 1 p.183 ~ p.189
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Antibacterial effects of amino acids-grafted water-soluble chitosan against drug-resistant bacteria
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Kim Jun-Ho
Kim Nam-Hong
Kim Eun-Ji
Kim Ji-Ho
Lee Min-Young
Park Yung-Hoon
Lee Jung-Ro
Park Seong-Cheol
Jang Mi-Kyeong
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Abstract
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Chitosan is an available natural polysaccharide that is biocompatible, biodegradable, and non-toxic. In this study, to enhance the antibacterial activity of water-soluble chitosan (WSC), amino acids with hydrophobic, anionic, or cationic side chains were grafted to the amine groups of WSC (amino acid-grafted WSC; AGW). Chemical characterization of AGW was performed by 1H nuclear magnetic resonance spectroscopy, resulting in grafting molecular percentages of 7.3 ~ 9.9%. AGW exhibited more potent antibacterial activity than unmodified WSC in both drug-susceptible and drug-resistant bacteria. Scanning electron microscopy and a calcein leakage assay in bacteria and artificial liposomes, respectively, revealed the antibacterial action of AGW to be membranolytic. Lysine or isoleucinegrafted WSC showed potent in vivo antibacterial activity in a drug-resistant Pseudomonas aeruginosa-infected mouse model. AGW has potential use as an effective antibacterial agent.
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KEYWORD
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water-soluble chitosan, drug-resistant bacteria, antibacterial activity, amino acid-graft, Pseudomonas aeruginosa
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